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Biodegradation of sulphonated azo dye C.I. reactive orange 16 by Enterococcus faecalis strain YZ 66

Madhuri M.Sahasrabudhe and Girish R. Pathade

Textile dye Reactive orange 16 was selected for biotransformation studies by Enterococcus faecalis YZ 66.Optimization of parameters for dye decolourization were studied under static anoxic condition. Under optimized condition decolourization of Reactive orange 16 by Enterococcus faecalis YZ 66 was found to be 77.73% in 80 minutes. Degradation of the dye was confirmed by UV- Visible spectophotometric, TLC, and HPLC analysis. The isolate had potential to decolourize mixture of five dyes. This indigenous isolate could be a potential organism for bioremediation of textile wastewater carrying dyes.