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Cytotoxic Effects of Titanium Dioxide Nanoparticles on Rat Embryo Fibroblast REF-3 Cell Line in vitro

Dhamia K. Suker and Reyam Moaid Albadran

Titanium dioxide nanoparticles (TiO₂ NPs) is an important industrial material that is widely used as an additive in cosmetics, pharmaceuticals, and food colorants. Although the small size of the TiO₂ nanoparticles is useful in various applications, the biosafety of this material needs to be estimated .For this purpose this study was designed to evaluated the cytotoxic effects of TiO₂nanoparticle on Rat Embryo Fibroblast cell line (REF-3) in vitro. Seven concentrations of TiO₂ nanoparticles (0.5,1,5,10,25,50 and100 ) μg/ml were prepared and tested on cell line with three replicates for each concentration ,The optical density of cell growth read by Elisa reader 492 nm by used Tetra zolium Bromide (MTT).The result of REF-3cellgrowth assay had shown that the TiO₂nanoparticles had cytotoxic inhibition ,This inhibition was time and dose dependent. the inhibitory effect of TiO₂nanoparticles on the proliferation of REF-3started from concentration 1 μg/ml during 24 hour .The CC50% of TiO₂nanoparticles on REF-3 cell line were more than 100μg/ml for all period of time