Ubaid Rasheed*, Faiza Momin, Hikmat Ullah, Imad Ali, Haq Nawaz, Latif Ahmad, Abid Khan and Mahideen Afridi
Background
Antimicrobial resistance is a challenging threat to global health. The resistance developed due to misuse or overuse of antibiotics. Antimicrobial resistance reduces the progress of antibiotics which significantly threaten human health. The study aims to explore antimicrobial activity of Pseudomonas MK-13 as well as optimization of different parameters essential for growth and antimicrobial activity.
Material and Methods
Antibacterial and antifungal activity of Pseudomonas MK-13 was checked against MDR bacterial strains (Acinetobacter, MRSA, E.coli and Pseudomonas) and fungi (Alternaria and Fusarium) by agar well diffusion method and dual culture technique respectively. Growth and antibiotic production activity were checked at different parameters i-e temperature, pH, incubation time and aeration.
Results
Interpretation of results showed that Pseudomonas MK-13 have significant antibacterialactivityagainstMRSA(17.5mm)andE.coli(16mm)followedbyAcinetobacter (11.7 mm) and Pseudomonas (10 mm). Pseudomonas MK-13 showed potential antifungal activity by significantly reducing growth of Alternaria and Fusarium by 16% and 14% respectively. Pseudomonas MK-13 has significant growth and antibacterial activity at PH 7, Temperature 37°C and at shaking aeration respectively. While negligible growth and activity were observed at PH 3, temperature 4°C and static aeration.
Conclusion
Highest growth and antibacterial activity of Pseudomonas MK-13 was found at PH 7, Temperature 37 °C and at shaking aeration. it is concluded that Pseudomonas MK-13 have significant antibacterial activity at PH 7, Temperature 37°C and at shaking aeration as well as potential antifungal activity. It is a beneficial parameter to produce antibacterial compounds to reduce the cost of production of potential compounds.
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