Европейский журнал экспериментальной биологии Открытый доступ

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Extraction of Ziziphora tenuior essential oil using supercritical CO₂

Tayebeh Darbandia, Bizhan Honarvarb, Mojtaba Sinaei Nobandeganic and Ardeshir Rezaeid

Ziziphora tenuior is an edible medicinal plant which belongs to Labiatae family. It is often used as a treatment for some diseases such as edema, insomnia, and hypertension in Turkey, Iran and China. In this study, the essential oil of Ziziphora tenuior was extracted by using a technique called ‘supercritical fluid extraction’. In addition, the components of the essential oil were identified by chromatography analysis. The main components of the essential oil were 53.977% of p-mentha-3-en-8-ol and 38.481% of pulegone. It must be noted that CO₂ was used as the supercritical fluid because it has a moderate critical temperature and pressure. In this study, the effects of some extraction parameter variations on the extraction yield were examined. Amongst the said parameters were pressure, temperature and mean particle size.