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FACT Creates a Transiently Accessible Nucleosome Structure Through Integrated Reorganization Mechanism

Yasuo Tsunaka and Kosuke Morikawa

Nucleosomes act as a barrier in nuclear processes, such as DNA transcription, replication, and repair. Facilitates chromatin transcription (FACT) plays essential roles in nucleosome dynamics during these processes. Two different mechanisms so far have been proposed for nucleosome reorganization by FACT. However, these interpretations have left some unsolved problems in terms of FACTmediated eviction and exchange of histones within nucleosome. Most recently, we have proposed the integrated reorganization mechanism, in which FACT retains nucleosome in transiently loosened structures. This accessible structure allows us to explain most reasonably about how FACT directs further disruption of nucleosome and histone replacement in concert with other factors.