Европейский журнал экспериментальной биологии Открытый доступ

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FemA gene in Indian isolates of methicillin resistant Staphylococcus aureus – isolation and amplification through real time PCR

Shashank Kr. Singh, Saleha Anwar, Hemant Kr. Sharma, Upendra Chaudhary and Sunil Kr. Verma

Statphylococcus aureus is a Gram positive bacterium, which is naturally present in nasopharyngeal region of human beings and animals. S. aureus had developed drug resistant capacity to against all penicillin class antibiotics after severe administration since 1941. Firstly, in 1961, Methicillin Resistant Staphylococcus aureus (MRSA) were isolated. In this research, Methicillin Resistant S. aureus (MRSA) were isolated from clinical pus samples. Isolates were grown on Luria Bertani (LB) Agar containing Oxacillin antibiotic by traditional methodologies. Genomic DNA was isolated from the isolates and electrophoresis was performed by using standard criteria. Fem A gene amplification was performed with Real Time PCR and with suitable primers. The amplified gene was produced 510 bp at Agarose Gel Electrophoresis.