Европейский журнал экспериментальной биологии Открытый доступ

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In-vitro callus induction from two different explants stem and leaf in Carthamus- tinctorius Linn.

Srikanti Kumari, R. K. Pandey and Uttam Kumar

In present study, callus was raised from two different explants stem and leaf of Carthamustinctorius. The explants were cultured from the 3 week old seedlings and transferred to an MS medium supplemented with different concentration of Auxin, 2, 4-D growth regulator hormones.The highest percentage of callus induction (90%) was observed in all surfaces of leaf explants.The medium supplemented with 2, 4-D was more effective that IAA for callus formation.