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Influence of interferon beta-1a on neurons of central nervous system and immune organs after initiation of demyelination and remyelination

Nataliia O. Melnyk

In experimental work was investigate morphological changers of neurons and myelinated nerve fibers in organs of central nervous system (CNS), organs of immune system - thymus and spleen, after initiation of experimental model of demyelination and remyelination. The model of demyelination - EAE (experimental allergic encephalomyelitis) in rats, was use to investigation of changers of neurons in cortex of cerebrum, cerebellum and spinal cord on 21 days and 39 days. After staining of histological sections of the brain and spinal cord by toluidine blue and cresyl violet, we observed the percentage of neurons with unmodified, moderate and severe structural changes. We studied of demyelination process of nervous fibers in organs of CNS by the methods of electron microscopy and morphometry. After influence of Rebif® (interferon beta- 1a) by 2 weeks, we observed of remyelination process - the percentage of normal neurons in the brain and spinal cord was increased, the amounts of neurons with severe and destructive changes were reduce and myelinated nerve fibers was regenerate. In thymus and spleen was observe structure changes – formation large nodules in spleen, activations of thymus – formation of small nodules in cortical parts of lobules. Was observe correlations between structural changes in organs of CNS and organs of immune system.