Европейский журнал экспериментальной биологии Открытый доступ

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Isolation and screening of endophytic actinomycetes producing antibacterial compound from Citrus aurantifolia Fruit

Kumud Chandra Kandpa, D. A. Jain, Umesh Kumar, Rashmi Tripathi and T Siva Kumar

Today more than 30,000 diseases are clinically described. Less than one third of these can be treated symptomatically and only a few can be cured. Actinomyctes is a potential producer of many antibiotics. Endophytic actinomycetes have been defined as that can be isolated from the disinfected surfaces of plant tissues or that can be extracted from within the plant that do not cause visible harm to the host It is noteworthy that, of the nearly 300,000 plant species that exist on the earth, In our study we have isolated the endophytic actinomycetes from citrus fruit. The total 5 actinomycetes were isolated from citrus fruits (Citrus aurantifolia) using Starch Casein agar, Out of 5 actinomycetes strains, 2 actinomycetes showing antibacterial activity were recovered using Bennet agar media. The production of antibacterial compound was performed using L.B. Broth. Out of 2 actinomycetes strain only one show (CT1) shows the strong antibacterial activity and fermentation carried out using L.B. Broth for 15 days. After fermentation, extraction of the supernatant was carried out using solvent petroleum ether. The Antibacterial compound was recovered using TLC and Column Chromatography. The Rf value of the compound was found to be (0.6152 ). The structural study of the extracted compound was carried out by UV-spectroscopy, FT-IR. The antibacterial compound was effective against E. coli, S. typhi, K. pneumoniae, S. aureus bacteria some of them got resistance against some antibiotic drug.