Европейский журнал экспериментальной биологии Открытый доступ

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Isolation, characterization and identification of bacterial biosurfactant

Mahalingam P.U and Nithya Sampath

Biosurfactants are extracellular, amphiphilic compounds produced by microorganisms as secondary metabolites which has a wide variety of application in food, pharmaceutical and cosmetics as well as in bioremediation purpose. In this study, two potential biosurfactant producing bacterial strains were isolated from diesel oil contaminated soil sites in Dindigul ,Tamilnadu, India and were identified as Pseudomonas sp and Bacillus sp. The biosurfactant production from the two bacterial strains were determined by standard qualitative methods. Both the bacterial strains has better emulsifying activity and surface tension reduction. Based on the chemical and FT-IR spectra analysis the biosurfactant compound produced by the Pseudomonas sp and Bacillus sp were identified as glycoprotein and lipoprotein respectively.