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Micropropagation of Chlorophytum borivilliens through direct organogenesis

Manisha Sharan, Indira L. Dhumne, Madhuri Sharon

Commercially viable protocol for in vitro propagation of Chlorophytum borivilliens was achieved through direct organogenesis from shoot apex explants. Cultures were initiated on MS medium supplemented with 2 mg l-1 benzyl adenine (BA). From one shoot apex, 250 shoots proliferated when it was transferred to MS medium supplemented with 3 mg l-1 benzyl adenine in six months time. Ninety eight percent of the regenerated shoots rooted within a month on MS medium supplemented with 0.05 mg l-1 naphthalene acetic acid. Rooted plants showed 100 % survival on transfer to in vivo conditions. From one plant in 7 months, 2200 plants were micropropagated and ready for field transfer.