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Production of α-amylase using banana waste by Bacillus subtilis under solid state fermentation

Chandrashekhar Unakal,Radha I. Kallur and Basappa B. Kaliwal

Banana waste was used as a substrate for the production of amylase by Bacillus subtilis using solid state fermentation. The various process parameters were optimized, the incubation period, substrate concentration, pH and incubation temperature showed 24hrs, 50g, 7 pH and 35°C respectively. Peptone (0.2%) as a nitrogen sources showed maximum yield and the maximum enzyme activity showed in presence of in organic nutrients magnesium sulphate (MgSo4.7H2O), calcium chloride (CaCl2.2H2O) and di-hydrogen potassium phosphate (KH2PO4) were 0.02%, 0.04% and 0.4% respectively