Европейский журнал экспериментальной биологии Открытый доступ

Абстрактный

Production of recombinant NPRC10 protease in 14-L fermentation scale

Nguyen Hoang Loc, Nguyen Sy Cong, Do Van Giap, Nguyen Thi Quy Hoa, Hoang Tan Quang, Nguyen Duc Huy

Present investigation describes the production of neutral protease (NPRC10) by recombinant E. coli BL21 (DE3) through submerged culture in 14-L fermenter. Different cultural parameters such as aeration rate, agitation speed and inoculum size were optimized for the production of enzyme. It was found that the maximum production of NPRC10 was obtained after 44 h of batch fermentation at aeration rate of 4 L/min, agitation speed of 500 rpm and inoculum size of 1%. The highest activity of protease during the time course of batch fermentation was 103 unit/mL